Hotspot

ycf1-ndhF

SSC · 110449-110472 · 24 bp

Back to Chaerophyllum

Pi 0.259

MarkerSeek score 69.40

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 24
variable_sites: 5
pi: 0.259
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GAAGCTCATGAAGGCCTACC	GTGGGCGCATCTCTTCTTAT	55.0	50.0	58.0	57.8	147-185 bp	1.0	88.3
ycf1-ndhF_p2	GAAGCTCATGAAGGCCTACC	GGGGAATAAGAGGATTGGCC	55.0	55.0	58.0	57.7	267-305 bp	1.0	85.8
ycf1-ndhF_p3	GAAGCTCATGAAGGCCTACC	GGGGGAATAAGAGGATTGGC	55.0	55.0	58.0	57.7	268-306 bp	1.0	85.7
ycf1-ndhF_p4	GAAGCTCATGAAGGCCTACC	TGTTCGTTGGATCCCTAGGA	55.0	50.0	58.0	57.8	621-659 bp	1.0	84.7
ycf1-ndhF_p5	GAAGCTCATGAAGGCCTACC	CGTTGTTCGTTGGATCCCTA	55.0	50.0	58.0	57.6	624-662 bp	1.0	83.7