Hotspot

rpl20-rps12

LSC · 69084-69855 · 772 bp

Back to Chaerophyllum

Pi 0.0181
MarkerSeek score 70.20
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
772
variable_sites
21
pi
0.0181
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATTTCCCCGT CAGCGTCGAGGAACATGTAC 50.0 55.0 58.0 58.7 829-836 bp 1.0 76.8
rpl20-rps12_p2 TCCGAGCTATATTTCCCCGT AGCGTCGAGGAACATGTACT 50.0 50.0 58.0 58.8 828-835 bp 1.0 76.0
rpl20-rps12_p3 TCCGAGCTATATTTCCCCGT TCAGCGTCGAGGAACATGTA 50.0 50.0 58.0 58.8 830-837 bp 1.0 76.0
rpl20-rps12_p4 TCCGAGCTATATTTCCCCGT GCGTCGAGGAACATGTACTA 50.0 50.0 58.0 57.2 827-834 bp 1.0 75.9
rpl20-rps12_p5 TTGAATGAGCCCCTCGAAAG CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 883-890 bp 1.0 75.8