Hotspot

trnW-CCA-trnP-UGG

LSC · 66690-66822 · 133 bp

Back to Chaerophyllum

Pi 0.0401

MarkerSeek score 75.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 8
pi: 0.0401
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	214-222 bp	1.0	84.8
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	213-221 bp	1.0	84.4
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	206-214 bp	1.0	84.4
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	241-249 bp	1.0	83.9
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	240-248 bp	1.0	83.5