Hotspot

psbE-petL

LSC · 65005-66129 · 1125 bp

Back to Chaerophyllum

Pi 0.0154

MarkerSeek score 68.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1125
variable_sites: 25
pi: 0.0154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1224-1283 bp	1.0	85.1
psbE-petL_p2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1237-1296 bp	1.0	84.7
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1227-1286 bp	1.0	84.5
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.8	58.0	1147-1206 bp	1.0	84.4
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1240-1299 bp	1.0	84.1