Hotspot

atpH-atpI

LSC · 13250-14415 · 1166 bp

Back to Chaerophyllum

Pi 0.0237

MarkerSeek score 64.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 40
pi: 0.0237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTCTAATTGGTCAGGAGCGC	45.0	50.0	58.0	58.0	1510-1622 bp	1.0	85.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TGCTTTGGCTTTACTCACGT	45.0	45.0	58.0	58.0	1417-1529 bp	1.0	85.6
atpH-atpI_p3	CTCGCGATACCTTCTACAGC	TTCTAATTGGTCAGGAGCGC	55.0	50.0	58.0	58.0	1600-1712 bp	1.0	85.5
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CTAATTGGTCAGGAGCGCTT	45.0	50.0	58.0	58.0	1508-1620 bp	1.0	85.5
atpH-atpI_p5	CTCGCGATACCTTCTACAGC	CTAATTGGTCAGGAGCGCTT	55.0	50.0	58.0	58.0	1598-1710 bp	1.0	85.4