Hotspot

trnT-CGU-trnR-UCU

LSC · 9461-9577 · 117 bp

Back to Chaerophyllum

Pi 0.0456

MarkerSeek score 78.00

Primer available yes

Label trnT-CGU-trnR-UCU

Feature Metadata

label_name: trnT-CGU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 8
pi: 0.0456
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-CGU-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	200-215 bp	1.0	69.5
trnT-CGU-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	203-218 bp	1.0	69.2
trnT-CGU-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	206-221 bp	1.0	68.7
trnT-CGU-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	209-224 bp	1.0	68.4
trnT-CGU-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	204-219 bp	1.0	67.0