Hotspot

rps16-trnQ-UUG

LSC · 5657-6892 · 1236 bp

Back to Chaerophyllum

Pi 0.0213
MarkerSeek score 66.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1236
variable_sites
37
pi
0.0213
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1171-1355 bp 1.0 84.4
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1172-1356 bp 1.0 84.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 1182-1366 bp 1.0 83.6
rps16-trnQ-UUG_p4 TGCTTTCTACCACAGCGTTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1135-1319 bp 1.0 81.8
rps16-trnQ-UUG_p5 TTGCTTTCTACCACAGCGTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1136-1320 bp 1.0 81.8