Hotspot

trnH-GUG-psbA

LSC · 74-254 · 181 bp

Back to Chaerophyllum

Pi 0.0652

MarkerSeek score 87.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 17
pi: 0.0652
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	247-248 bp	1.0	84.6
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	297-298 bp	1.0	83.8
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	311-312 bp	1.0	83.6
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	310-311 bp	1.0	83.6
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	312-313 bp	1.0	83.5