primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	74	254	247-248	1.000000	84.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	74	254	297-298	1.000000	83.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	74	254	311-312	1.000000	83.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	74	254	310-311	1.000000	83.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	74	254	312-313	1.000000	83.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TGCTTTCTACCACAGCGTTT	AAATATAGATGGGGCGTGGC	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.238579	5657	6892	1171-1355	1.000000	84.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TTGCTTTCTACCACAGCGTT	AAATATAGATGGGGCGTGGC	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.238579	5657	6892	1172-1356	1.000000	84.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	AAATATAGATGGGGCGTGGC	20	20	45.0	50.0	58.1	57.8	8.1	0.0	0.339697	5657	6892	1182-1366	1.000000	83.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TGCTTTCTACCACAGCGTTT	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.571350	5657	6892	1135-1319	1.000000	81.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTGCTTTCTACCACAGCGTT	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.571350	5657	6892	1136-1320	1.000000	81.8
trnT-CGU_p1	trnT-CGU	1	ATTTTGTTCGAAAGGGCCCT	CCCTCTTGCGGTAGAAGAAC	20	20	45.0	55.0	58.0	58.0	22.3	0.0	0.021222	8683	9460	1663-1711	1.000000	83.5
trnT-CGU_p2	trnT-CGU	2	ATTTTGTTCGAAAGGGCCCT	GAGGAAGCAGAAGCCCTTTT	20	20	45.0	50.0	58.0	58.1	22.3	0.0	0.093176	8683	9460	1494-1542	1.000000	83.3
trnT-CGU_p3	trnT-CGU	3	ATTAGCAATCCGCCGCTTTA	CCCTCTTGCGGTAGAAGAAC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.049747	8683	9460	1884-1932	1.000000	83.1
trnT-CGU_p4	trnT-CGU	4	ATTAGCAATCCGCCGCTTTA	GAGGAAGCAGAAGCCCTTTT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.121701	8683	9460	1715-1763	1.000000	82.8
trnT-CGU_p5	trnT-CGU	5	ACTCAGCCATCTCTCCCAAT	CCCTCTTGCGGTAGAAGAAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.120988	8683	9460	1860-1908	1.000000	82.6
trnT-CGU-trnR-UCU_p1	trnT-CGU-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9461	9577	200-215	1.000000	69.5
trnT-CGU-trnR-UCU_p2	trnT-CGU-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9461	9577	203-218	1.000000	69.2
trnT-CGU-trnR-UCU_p3	trnT-CGU-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9461	9577	206-221	1.000000	68.7
trnT-CGU-trnR-UCU_p4	trnT-CGU-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9461	9577	209-224	1.000000	68.4
trnT-CGU-trnR-UCU_p5	trnT-CGU-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9461	9577	204-219	1.000000	67.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTCTAATTGGTCAGGAGCGC	20	20	45.0	50.0	58.0	58.0	1.4	0.0	0.062562	13250	14415	1510-1622	1.000000	85.6
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TGCTTTGGCTTTACTCACGT	20	20	45.0	45.0	58.0	58.0	1.4	0.0	0.076593	13250	14415	1417-1529	1.000000	85.6
atpH-atpI_p3	atpH-atpI	3	CTCGCGATACCTTCTACAGC	TTCTAATTGGTCAGGAGCGC	20	20	55.0	50.0	58.0	58.0	10.4	0.0	0.063502	13250	14415	1600-1712	1.000000	85.5
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	CTAATTGGTCAGGAGCGCTT	20	20	45.0	50.0	58.0	58.0	1.4	13.3	0.068225	13250	14415	1508-1620	1.000000	85.5
atpH-atpI_p5	atpH-atpI	5	CTCGCGATACCTTCTACAGC	CTAATTGGTCAGGAGCGCTT	20	20	55.0	50.0	58.0	58.0	10.4	13.3	0.069165	13250	14415	1598-1710	1.000000	85.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46962	47749	809-839	1.000000	84.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46962	47749	841-871	1.000000	83.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.9	57.8	0.0	15.0	0.338047	46962	47749	827-857	1.000000	82.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.8	57.8	15.5	15.0	0.445800	46962	47749	859-889	1.000000	81.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.527459	46962	47749	811-841	1.000000	81.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.060086	65005	66129	1224-1283	1.000000	85.1
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097701	65005	66129	1237-1296	1.000000	84.7
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	6.4	0.0	0.134501	65005	66129	1227-1286	1.000000	84.5
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.165095	65005	66129	1147-1206	1.000000	84.4
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.172116	65005	66129	1240-1299	1.000000	84.1
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	0.0	8.4	0.175650	66690	66822	214-222	1.000000	84.8
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	0.0	8.4	0.232908	66690	66822	213-221	1.000000	84.4
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.252065	66690	66822	206-214	1.000000	84.4
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	6.2	8.4	0.202860	66690	66822	241-249	1.000000	83.9
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	6.2	8.4	0.260118	66690	66822	240-248	1.000000	83.5
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATTTCCCCGT	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	58.0	58.7	0.0	9.8	0.736204	69084	69855	829-836	1.000000	76.8
rpl20-rps12_p2	rpl20-rps12	2	TCCGAGCTATATTTCCCCGT	AGCGTCGAGGAACATGTACT	20	20	50.0	50.0	58.0	58.8	0.0	9.8	0.830587	69084	69855	828-835	1.000000	76.0
rpl20-rps12_p3	rpl20-rps12	3	TCCGAGCTATATTTCCCCGT	TCAGCGTCGAGGAACATGTA	20	20	50.0	50.0	58.0	58.8	0.0	9.8	0.834928	69084	69855	830-837	1.000000	76.0
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATATTTCCCCGT	GCGTCGAGGAACATGTACTA	20	20	50.0	50.0	58.0	57.2	0.0	9.8	0.843756	69084	69855	827-834	1.000000	75.9
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	69084	69855	883-890	1.000000	75.8
psbT-psbN_p1	psbT-psbN	1	ACGATCGAATCTATGGAAGCA	AGAGATCCGTTCGAGGAACA	21	20	42.9	50.0	57.0	58.2	24.3	9.0	2.174347	74433	74523	231-235	1.000000	65.0
psbT-psbN_p2	psbT-psbN	2	ACGATCGAATCTATGGAAGCA	TCGAGGAACATGGGGACTAG	21	20	42.9	55.0	57.0	58.2	24.3	0.0	2.233180	74433	74523	221-225	1.000000	64.6
psbT-psbN_p3	psbT-psbN	3	ACGATCGAATCTATGGAAGCA	TTCGAGGAACATGGGGACTA	21	20	42.9	50.0	57.0	57.8	24.3	0.0	2.251122	74433	74523	222-226	1.000000	64.5
psbT-psbN_p4	psbT-psbN	4	ACCACGATCGAATCTATGGA	AGAGATCCGTTCGAGGAACA	20	20	45.0	50.0	55.8	58.2	24.3	9.0	2.317269	74433	74523	234-238	1.000000	63.8
psbT-psbN_p5	psbT-psbN	5	ACGATCGAATCTATGGAAGCA	GGGTACGCCTTATATACCGC	21	20	42.9	55.0	57.0	57.7	24.3	0.0	2.294104	74433	74523	276-280	1.000000	63.7
psbH-petB_p1	psbH-petB	1	AACAGCTCCTTTGATGGGTG	AGATGACAGACCCGTTCTCT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.308662	74980	75102	293-294	1.000000	78.9
psbH-petB_p2	psbH-petB	2	AACAGCTCCTTTGATGGGTG	TGACAGACCCGTTCTCTGTA	20	20	50.0	50.0	58.1	57.7	0.0	6.8	0.366398	74980	75102	290-291	1.000000	78.4
psbH-petB_p3	psbH-petB	3	AACAGCTCCTTTGATGGGTG	GATGACAGACCCGTTCTCTG	20	20	50.0	55.0	58.1	57.7	0.0	0.0	0.370686	74980	75102	292-293	1.000000	78.4
psbH-petB_p4	psbH-petB	4	AACAGCTCCTTTGATGGGTG	ACAGACCCGTTCTCTGTACT	20	20	50.0	50.0	58.1	57.7	0.0	5.3	0.374451	74980	75102	288-289	1.000000	78.4
psbH-petB_p5	psbH-petB	5	GAACAGCTCCTTTGATGGGT	AGATGACAGACCCGTTCTCT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.430380	74980	75102	294-295	1.000000	77.9
ycf1-ndhF_p1	ycf1-ndhF	1	GAAGCTCATGAAGGCCTACC	GTGGGCGCATCTCTTCTTAT	20	20	55.0	50.0	58.0	57.8	5.2	10.8	0.286302	110449	110472	147-185	1.000000	88.3
ycf1-ndhF_p2	ycf1-ndhF	2	GAAGCTCATGAAGGCCTACC	GGGGAATAAGAGGATTGGCC	20	20	55.0	55.0	58.0	57.7	5.2	0.0	0.334033	110449	110472	267-305	1.000000	85.8
ycf1-ndhF_p3	ycf1-ndhF	3	GAAGCTCATGAAGGCCTACC	GGGGGAATAAGAGGATTGGC	20	20	55.0	55.0	58.0	57.7	5.2	0.0	0.334033	110449	110472	268-306	1.000000	85.7
ycf1-ndhF_p4	ycf1-ndhF	4	GAAGCTCATGAAGGCCTACC	TGTTCGTTGGATCCCTAGGA	20	20	55.0	50.0	58.0	57.8	5.2	11.5	0.280864	110449	110472	621-659	1.000000	84.7
ycf1-ndhF_p5	ycf1-ndhF	5	GAAGCTCATGAAGGCCTACC	CGTTGTTCGTTGGATCCCTA	20	20	55.0	50.0	58.0	57.6	5.2	9.9	0.413124	110449	110472	624-662	1.000000	83.7
ndhF_p1	ndhF	1	GAAGCTCATGAAGGCCTACC	GGGGGTAGGATCCGGTAAAT	20	20	55.0	55.0	58.0	58.3	5.2	6.0	0.301110	110473	112707	2359-2397	1.000000	80.2
ndhF_p2	ndhF	2	GCTCATGAAGGCCTACCATT	GGGGGTAGGATCCGGTAAAT	20	20	50.0	55.0	57.6	58.3	5.2	6.0	0.616536	110473	112707	2356-2394	1.000000	77.7
ndhF_p3	ndhF	3	CGAAGCTCATGAAGGCCTAC	GGGGGTAGGATCCGGTAAAT	20	20	55.0	55.0	58.4	58.3	5.2	6.0	0.685171	110473	112707	2360-2398	1.000000	77.2
ndhF_p4	ndhF	4	AGCTCATGAAGGCCTACCAT	GGGGGTAGGATCCGGTAAAT	20	20	50.0	55.0	58.5	58.3	5.2	6.0	0.745417	110473	112707	2357-2395	1.000000	76.7
ndhF_p5	ndhF	5	GAAGCTCATGAAGGCCTACC	GGGTAGGATCCGGTAAATCG	20	20	55.0	55.0	58.0	57.3	5.2	6.0	0.777596	110473	112707	2357-2395	1.000000	76.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCCTTAAAAGCATTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	12.4	0.0	0.022166	113833	114600	807-896	1.000000	87.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCCTTAAAAGCATTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	12.4	0.0	0.023536	113833	114600	808-897	1.000000	87.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCCTTAAAAGCATTG	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.0	58.1	12.4	0.0	0.094417	113833	114600	828-917	1.000000	86.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCCTTAAAAGCATTG	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.0	58.1	12.4	0.0	0.094417	113833	114600	830-919	1.000000	86.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCCTTAAAAGCATTG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	12.4	0.0	0.138613	113833	114600	817-906	1.000000	86.4
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	TGGACCGCGAGAGTTATTTG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.012413	114775	115749	1475-1485	1.000000	82.4
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	TGGACCGCGAGAGTTATTTG	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.013783	114775	115749	1474-1484	1.000000	82.4
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	GGACCGCGAGAGTTATTTGT	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.013801	114775	115749	1474-1484	1.000000	82.4
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	GGACCGCGAGAGTTATTTGT	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.015171	114775	115749	1473-1483	1.000000	82.4
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	AAACGATACGGGACTCAACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.021132	114775	115749	1885-1885	0.333333	53.2
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	CGAACAATTTTGAATTTGCCTGA	20	23	50.0	34.8	58.0	57.1	0.0	0.0	3.951143	119144	119462	415-467	1.000000	53.2
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCGATCCCAGAAAGA	TCGAACAATTTTGAATTTGCCTG	20	23	50.0	34.8	58.0	57.1	0.0	0.0	3.951143	119144	119462	416-468	1.000000	53.2
ndhG-ndhI_p3	ndhG-ndhI	3	AAGACCCGATCCCAGAAAGA	ACTCGAACAATTTTGAATTTGCC	20	23	50.0	34.8	58.0	57.1	0.0	0.0	3.952858	119144	119462	418-470	1.000000	53.1
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCCGATCCCAGAAAGA	CTCGAACAATTTTGAATTTGCCTG	20	24	50.0	37.5	58.0	58.1	0.0	0.0	4.139272	119144	119462	417-469	1.000000	51.6
ndhG-ndhI_p5	ndhG-ndhI	5	AAGACCCGATCCCAGAAAGA	CTCGAACAATTTTGAATTTGCCT	20	23	50.0	34.8	58.0	56.8	0.0	0.0	4.208489	119144	119462	417-469	1.000000	51.1
ycf1_p1	ycf1	1	TTTTGGTTAGTTTCGCCGGA	AGAGTTTTAAAAGAAGGAACAAA	20	23	45.0	26.1	58.0	52.7	5.0	0.0	8.361761	126728	126847	179-179	1.000000	46.3
ycf1_p2	ycf1	2	TTTGGTTAGTTTCGCCGGAT	AAGAGTTTTAAAAGAAGGAACAA	20	23	45.0	26.1	57.8	52.7	5.0	0.0	8.514375	126728	126847	179-179	1.000000	46.3
ycf1_p3	ycf1	3	TTTGGTTAGTTTCGCCGGAT	AGAGTTTTAAAAGAAGGAACAAA	20	23	45.0	26.1	57.8	52.7	5.0	0.0	8.514375	126728	126847	178-178	1.000000	46.3
ycf1_p4	ycf1	4	TTTTGGTTAGTTTCGCCGGA	AGAGTTTTAAAAGAAGGAACAAAA	20	24	45.0	25.0	58.0	53.5	5.0	0.0	8.539060	126728	126847	179-179	1.000000	46.3
ycf1_p5	ycf1	5	TTTTGGTTAGTTTCGCCGGA	AAGAGTTTTAAAAGAAGGAACAA	20	23	45.0	26.1	58.0	52.7	5.0	0.0	8.361761	126728	126847	180-180	1.000000	46.2