Hotspot

rpl16

LSC · 79757-81039 · 1283 bp

Back to Chaenorhinum

Pi 0.00156

MarkerSeek score 59.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1283
variable_sites: 2
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCCAAGACTCATTTCTGTCG	CACTTCATTCTTTTTCTGGCCA	47.6	40.9	58.0	57.4	1417 bp	1.0	51.0
rpl16_p2	CCAAGACTCATTTCTGTCGGT	CACTTCATTCTTTTTCTGGCCA	47.6	40.9	58.0	57.4	1415 bp	1.0	51.0
rpl16_p3	ACCCAAGACTCATTTCTGTCG	ACACTTCATTCTTTTTCTGGCC	47.6	40.9	58.0	57.4	1418 bp	1.0	51.0
rpl16_p4	CCAAGACTCATTTCTGTCGGT	ACACTTCATTCTTTTTCTGGCC	47.6	40.9	58.0	57.4	1416 bp	1.0	51.0
rpl16_p5	CCAAGACTCATTTCTGTCGGT	ACTTCATTCTTTTTCTGGCCA	47.6	38.1	58.0	56.0	1414 bp	1.0	47.4