Hotspot

psbD

LSC · 31841-32902 · 1062 bp

Back to Chaenorhinum

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	CTTCAGACGCCCCGAAAATA	AACCCGTGGTTTCTTGGTC	50.0	52.6	58.0	57.9	1174 bp	1.0	71.1
psbD_p2	TGATCGAATCTTCAGACGCC	AACCCGTGGTTTCTTGGTC	50.0	52.6	57.8	57.9	1183 bp	1.0	69.5
psbD_p3	TTCAGACGCCCCGAAAATAC	AACCCGTGGTTTCTTGGTC	50.0	52.6	58.3	57.9	1173 bp	1.0	69.1
psbD_p4	GCTCGGAAATGGTCGAAGTA	AACCCGTGGTTTCTTGGTC	50.0	52.6	57.7	57.9	1145 bp	1.0	68.9
psbD_p5	GGGCAGTGCAAGAGAAATCA	AACCCGTGGTTTCTTGGTC	50.0	52.6	58.5	57.9	1211 bp	1.0	67.5