Hotspot

psbB

LSC · 71105-72631 · 1527 bp

Back to Chaenorhinum

Pi 0.000655

MarkerSeek score 47.30

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 1
pi: 0.000655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	ACTGAACAAGGGAGGTCCAT	GGCGAAAAATACCAAAGCAGT	50.0	42.9	58.3	57.7	1654 bp	1.0	67.6
psbB_p2	ATCCACTGAACAAGGGAGGT	GGCGAAAAATACCAAAGCAGT	50.0	42.9	58.3	57.7	1658 bp	1.0	67.6
psbB_p3	ACTGAACAAGGGAGGTCCAT	GAGGCGAAAAATACCAAAGCA	50.0	42.9	58.3	57.4	1656 bp	1.0	65.5
psbB_p4	ATCCACTGAACAAGGGAGGT	GAGGCGAAAAATACCAAAGCA	50.0	42.9	58.3	57.4	1660 bp	1.0	65.5
psbB_p5	ACTGAACAAGGGAGGTCCAT	AGGCGAAAAATACCAAAGCAG	50.0	42.9	58.3	57.4	1655 bp	1.0	65.4