Hotspot

psbA

LSC · 314-1372 · 1059 bp

Back to Chaenorhinum

Pi 0

MarkerSeek score 29.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTGCTCCTTTATTTTCCCGA	AAATTTCCGTGCTTGGGAGT	40.0	45.0	54.9	58.0	1168 bp	1.0	55.5
psbA_p2	TTGCTCCTTTATTTTCCCGA	GTGCTTGGGAGTCTCTGATG	40.0	55.0	54.9	58.0	1160 bp	1.0	55.3
psbA_p3	TTGCTCCTTTATTTTCCCGA	TGCTTGGGAGTCTCTGATGA	40.0	50.0	54.9	58.1	1159 bp	1.0	55.1
psbA_p4	TGCTCCTTTATTTTCCCGATTCT	AAATTTCCGTGCTTGGGAGT	39.1	45.0	58.1	58.0	1167 bp	1.0	54.8
psbA_p5	TGCTCCTTTATTTTCCCGATTCT	GTGCTTGGGAGTCTCTGATG	39.1	55.0	58.1	58.0	1159 bp	1.0	54.7