Hotspot

petA

LSC · 60950-61912 · 963 bp

Back to Chaenorhinum

Pi 0.00104

MarkerSeek score 59.30

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGCAGAATCGTGTATAGGGAAC	CTTGATGTCGATCAATTCGCG	45.5	47.6	57.7	58.1	1064 bp	1.0	52.6
petA_p2	AGCAGAATCGTGTATAGGGA	CTTGATGTCGATCAATTCGCG	45.0	47.6	55.4	58.1	1064 bp	1.0	50.7
petA_p3	AAGCAGAATCGTGTATAGGGA	CTTGATGTCGATCAATTCGCG	42.9	47.6	56.2	58.1	1065 bp	1.0	49.1
petA_p4	AGCAGAATCGTGTATAGGGAA	CTTGATGTCGATCAATTCGCG	42.9	47.6	56.2	58.1	1064 bp	1.0	49.1
petA_p5	GCAGAATCGTGTATAGGGAAC	CTTGATGTCGATCAATTCGCG	47.6	47.6	56.2	58.1	1063 bp	1.0	49.0