Hotspot

psbB-psbT

LSC · 72632-72799 · 168 bp

Back to Chaenorhinum

Pi 0.0119

MarkerSeek score 59.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 2
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TGGCGCATTCCAAAAACTTG	TGATCCCTAGAGTCGAGACT	45.0	50.0	58.1	56.3	269 bp	1.0	65.9
psbB-psbT_p2	TAGAGTTTGGCGCATTCCAA	TGATCCCTAGAGTCGAGACT	45.0	50.0	57.8	56.3	276 bp	1.0	65.4
psbB-psbT_p3	CTCAAGTAGAGTTTGGCGCA	TGATCCCTAGAGTCGAGACT	50.0	50.0	58.2	56.3	282 bp	1.0	65.3
psbB-psbT_p4	TGCTCAAGTAGAGTTTGGCG	TGATCCCTAGAGTCGAGACT	50.0	50.0	58.2	56.3	284 bp	1.0	65.3
psbB-psbT_p5	AGTAGAGTTTGGCGCATTCC	TGATCCCTAGAGTCGAGACT	50.0	50.0	58.3	56.3	278 bp	1.0	64.9