Hotspot

ndhC-trnV-UAC

LSC · 49491-50608 · 1118 bp

Back to Chaenorhinum

Pi 0.0027

MarkerSeek score 60.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1118
variable_sites: 3
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1242-1249 bp	1.0	78.4
ndhC-trnV-UAC_p2	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1263-1270 bp	1.0	75.8
ndhC-trnV-UAC_p3	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1241-1248 bp	1.0	75.7
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1230-1237 bp	1.0	74.5
ndhC-trnV-UAC_p5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.6	57.8	1255-1262 bp	1.0	74.5