Hotspot

ycf3-trnS-GGA

LSC · 43406-44214 · 809 bp

Back to Chaenorhinum

Pi 0.00247
MarkerSeek score 60.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
809
variable_sites
2
pi
0.00247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 981 bp 1.0 72.5
ycf3-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 980 bp 1.0 72.5
ycf3-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 1002 bp 1.0 72.0
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 1001 bp 1.0 72.0
ycf3-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 952 bp 1.0 71.7