Hotspot

rps16-trnQ-UUG

LSC · 6171-6855 · 685 bp

Back to Chaenorhinum

Pi 0.00292
MarkerSeek score 60.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
685
variable_sites
2
pi
0.00292
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 775 bp 1.0 79.0
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG CGAAATTGAAATGGGGCGTC 50.0 50.0 57.9 58.1 834 bp 1.0 78.8
rps16-trnQ-UUG_p3 AACGGATCGTGTCCTTCAAG GAAATTGAAATGGGGCGTCG 50.0 50.0 57.9 58.1 833 bp 1.0 78.8
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 758 bp 1.0 78.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTCG 45.0 50.0 58.1 58.1 816 bp 1.0 78.3