Hotspot

psaI

LSC · 62745-62858 · 114 bp

Back to Chaenomeles

Pi 0.00439

MarkerSeek score 41.50

Primer available yes

Label psaI

Feature Metadata

label_name: psaI
feature_type: gene
region: LSC
length_bp: 114
variable_sites: 1
pi: 0.00439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI_p1	TAAATCGAGGTACCCATTCT	CGAAGGAAGCCGCATATTAT	40.0	45.0	53.1	55.8	242 bp	1.0	40.3
psaI_p2	TTAAATCGAGGTACCCATTCT	CGAAGGAAGCCGCATATTAT	38.1	45.0	54.0	55.8	243 bp	1.0	40.3
psaI_p3	TAAATCGAGGTACCCATTCT	AGGAAGCCGCATATTATACCA	40.0	42.9	53.1	56.7	239 bp	1.0	40.3
psaI_p4	TTAAATCGAGGTACCCATTCT	AGGAAGCCGCATATTATACCA	38.1	42.9	54.0	56.7	240 bp	1.0	40.3
psaI_p5	TAAATCGAGGTACCCATTCT	AAGGAAGCCGCATATTATACCA	40.0	40.9	53.1	57.4	240 bp	1.0	40.3