Hotspot

accD

LSC · 60380-61906 · 1527 bp

Back to Chaenomeles

Pi 0.00131

MarkerSeek score 40.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCCTAGTGATTGGATTTCTATGCT	AACGACTTGATCACCACACA	41.7	45.0	58.1	57.4	1678-1702 bp	1.0	42.2
accD_p2	CCCCTCGTTATTAGTTACTAACCC	AACGACTTGATCACCACACA	45.8	45.0	58.2	57.4	1699-1723 bp	1.0	42.0
accD_p3	CCCTAGTGATTGGATTTCTATGC	AACGACTTGATCACCACACA	43.5	45.0	56.8	57.4	1678-1702 bp	1.0	41.6
accD_p4	CCCTAGTGATTGGATTTCTATGCT	ACGACTTGATCACCACACAT	41.7	45.0	58.1	57.2	1677-1701 bp	1.0	40.4
accD_p5	ACCCTAGTGATTGGATTTCTATGC	AACGACTTGATCACCACACA	41.7	45.0	58.4	57.4	1679-1703 bp	1.0	40.2