Hotspot

petA-psbJ

LSC · 66221-67276 · 1056 bp

Back to Chaenomeles

Pi 0

MarkerSeek score 42.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1204-1227 bp	1.0	75.1
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1205-1228 bp	1.0	75.1
petA-psbJ_p3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.7	57.8	1220-1243 bp	1.0	74.2
petA-psbJ_p4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.3	57.8	1211-1234 bp	1.0	74.0
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.2	57.8	1227-1250 bp	1.0	73.6