Hotspot

atpH-atpI

LSC · 14346-15509 · 1164 bp

Back to Chaenomeles

Pi 0.00115

MarkerSeek score 40.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 2
pi: 0.00115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1256-1257 bp	1.0	79.7
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1222-1223 bp	1.0	79.6
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	50.0	45.0	58.0	57.9	1286-1287 bp	1.0	78.8
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1252-1253 bp	1.0	78.8
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	1223-1224 bp	1.0	78.4