Hotspot

rps16-trnQ-UUG

LSC · 6352-7178 · 827 bp

Back to Chaenomeles

Pi 0.00484

MarkerSeek score 43.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 6
pi: 0.00484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	911-913 bp	1.0	78.3
rps16-trnQ-UUG_p2	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	921-923 bp	1.0	76.6
rps16-trnQ-UUG_p3	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	888-890 bp	1.0	76.6
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	922-924 bp	1.0	76.5
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	50.0	55.0	58.2	58.3	973-975 bp	1.0	76.1