Hotspot

ycf1

IRb · 110496-116453 · 5958 bp

Back to Ceropegia

Pi 0.00112

MarkerSeek score 55.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5958
variable_sites: 13
pi: 0.00112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTTGCTGCTTAGACTGCA	TGCGATAAAATGGTTTCGGG	45.0	45.0	58.0	56.8	293 bp	1.0	70.5
ycf1_p2	TGTTTGCTGCTTAGACTGCA	TGCGATAAAATGGTTTCGGGA	45.0	42.9	58.0	58.3	293 bp	1.0	70.0
ycf1_p3	TGTTTGCTGCTTAGACTGCA	GCGATAAAATGGTTTCGGGA	45.0	45.0	58.0	56.5	292 bp	1.0	68.3
ycf1_p4	TGTTTGCTGCTTAGACTGCA	GCTTCATCTTATGAGCCAACG	45.0	47.6	58.0	57.4	223 bp	1.0	67.7
ycf1_p5	TGTTTGCTGCTTAGACTGCA	ATGCGATAAAATGGTTTCGGG	45.0	42.9	58.0	57.3	294 bp	1.0	66.6