Hotspot

rpoC2

LSC · 16771-20877 · 4107 bp

Back to Ceropegia

Pi 0.00256

MarkerSeek score 55.70

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4107
variable_sites: 21
pi: 0.00256
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGCAGGGAAAAGTGTTGCTA	CATAGCGGCGTTTTGATACC	45.0	50.0	57.3	57.3	183 bp	1.0	70.7
rpoC2_p2	AGCAGGGAAAAGTGTTGCTA	CCATAGCGGCGTTTTGATAC	45.0	50.0	57.3	57.3	184 bp	1.0	70.7
rpoC2_p3	TGCAACATATCAGCAGGGAA	CATAGCGGCGTTTTGATACC	45.0	50.0	57.2	57.3	194 bp	1.0	69.5
rpoC2_p4	TGCAACATATCAGCAGGGAA	CCATAGCGGCGTTTTGATAC	45.0	50.0	57.2	57.3	195 bp	1.0	69.5
rpoC2_p5	CAGCAGGGAAAAGTGTTGCT	CATAGCGGCGTTTTGATACC	50.0	50.0	59.0	57.3	184 bp	1.0	68.3