Hotspot

ndhF

SSC · 116927-119170 · 2244 bp

Back to Ceropegia

Pi 0.0078

MarkerSeek score 40.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 35
pi: 0.0078
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGGGGGTCGGTTGAATTTC	AGGAAATTCACAAAGCTTTTT	50.0	28.6	58.1	52.6	2315-2336 bp	1.0	41.4
ndhF_p2	GAAGGGGGTCGGTTGAATTT	AGGAAATTCACAAAGCTTTTT	50.0	28.6	58.1	52.6	2316-2337 bp	1.0	41.4
ndhF_p3	AAAAGAAAGAAGGGGGTCGG	AGGAAATTCACAAAGCTTTTT	50.0	28.6	57.7	52.6	2324-2345 bp	1.0	41.4
ndhF_p4	GGGGGTCGGTTGAATTTCAA	AGGAAATTCACAAAGCTTTTT	50.0	28.6	58.4	52.6	2313-2334 bp	1.0	41.4
ndhF_p5	AAGAAAGAAGGGGGTCGGTT	AGGAAATTCACAAAGCTTTTT	50.0	28.6	58.9	52.6	2322-2343 bp	1.0	41.4