Hotspot

ndhD-psaC

SSC · 123896-124013 · 118 bp

Back to Ceropegia

Pi 0.0212

MarkerSeek score 53.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 118
variable_sites: 5
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAGTATACCAACGAACGGCC	GGAAGACTGCGTTGGTTGTA	50.0	50.0	58.0	58.1	339-340 bp	1.0	80.1
ndhD-psaC_p2	AACCCGCAGCTATTGGAAAA	GGAAGACTGCGTTGGTTGTA	45.0	50.0	58.1	58.1	285-286 bp	1.0	79.7
ndhD-psaC_p3	ACCCGCAGCTATTGGAAAAA	GGAAGACTGCGTTGGTTGTA	45.0	50.0	58.1	58.1	284-285 bp	1.0	79.7
ndhD-psaC_p4	AACGGCCCTATTTCCCCTAT	GGAAGACTGCGTTGGTTGTA	50.0	50.0	58.2	58.1	326-327 bp	1.0	78.8
ndhD-psaC_p5	AAATTAAGGAACCCGCAGCT	GGAAGACTGCGTTGGTTGTA	45.0	50.0	57.8	58.1	294-295 bp	1.0	78.6