Hotspot

rpl32-trnL-UAG

SSC · 120088-120997 · 910 bp

Back to Ceropegia

Pi 0.0132
MarkerSeek score 61.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
910
variable_sites
23
pi
0.0132
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACAGGAAATTCAAAAAGCTTT AGCAGCGTGTCTACCAATTT 28.6 45.0 52.6 57.8 836-984 bp 1.0 46.0
rpl32-trnL-UAG_p2 ACAGGAAATTCAAAAAGCTTT TCCTAAGAGCAGCGTGTCTA 28.6 50.0 52.6 57.9 843-991 bp 1.0 45.9
rpl32-trnL-UAG_p3 ACAGGAAATTCAAAAAGCTTT CCTAAGAGCAGCGTGTCTAC 28.6 55.0 52.6 57.8 842-990 bp 1.0 45.9
rpl32-trnL-UAG_p4 ACAGGAAATTCAAAAAGCTTT CTAAGAGCAGCGTGTCTACC 28.6 55.0 52.6 57.8 841-989 bp 1.0 45.9
rpl32-trnL-UAG_p5 ACAGGAAATTCAAAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 27.3 50.0 53.5 57.9 843-991 bp 1.0 45.9