Hotspot

psbE-petL

LSC · 66240-67376 · 1137 bp

Back to Ceropegia

Pi 0.00718

MarkerSeek score 61.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1137
variable_sites: 15
pi: 0.00718
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCA	45.0	45.0	58.0	56.9	1295-1314 bp	1.0	71.8
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCCGCTAGTAGAAAACCAA	45.0	45.0	58.0	56.9	1294-1313 bp	1.0	71.8
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1284-1303 bp	1.0	71.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1283-1302 bp	1.0	71.5
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1206-1225 bp	1.0	70.7