Hotspot

psaI-ycf4

LSC · 61190-61626 · 437 bp

Back to Ceropegia

Pi 0.00229

MarkerSeek score 53.20

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 2
pi: 0.00229
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	579-586 bp	1.0	79.0
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	582-589 bp	1.0	79.0
psaI-ycf4_p3	CAGTCTATGAACCTTCCCGC	GGATAAAGGCCCAGCAGAAA	55.0	50.0	58.1	57.9	626-633 bp	1.0	78.8
psaI-ycf4_p4	CAGTCTATGAACCTTCCCGC	AAAGGATAAAGGCCCAGCAG	55.0	50.0	58.1	57.9	629-636 bp	1.0	78.7
psaI-ycf4_p5	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	50.0	50.0	57.9	57.9	589-596 bp	1.0	78.5