Hotspot

psaA-ycf3

LSC · 43057-44094 · 1038 bp

Back to Ceropegia

Pi 0.013

MarkerSeek score 58.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1038
variable_sites: 27
pi: 0.013
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCTGCCGAGTATTGG	50.0	50.0	58.1	58.0	1235-1254 bp	1.0	83.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	1216-1235 bp	1.0	83.4
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	1164-1183 bp	1.0	82.8
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	CGATCAAGCTGCCGAGTATT	50.0	50.0	58.1	58.1	1237-1256 bp	1.0	82.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CGATCAAGCTGCCGAGTATT	50.0	50.0	57.9	58.1	1218-1237 bp	1.0	82.6