Hotspot

trnE-UUC-trnT-GGU

LSC · 31785-32740 · 956 bp

Back to Ceropegia

Pi 0.0115

MarkerSeek score 48.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 956
variable_sites: 22
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TAGATTGGCGAATTGTGGGG	ACCGGAGTTTTATGGAAGCC	50.0	50.0	57.9	57.9	1266-1344 bp	1.0	82.8
trnE-UUC-trnT-GGU_p2	TAGATTGGCGAATTGTGGGG	CGCATCCATTCTCTATCGCA	50.0	50.0	57.9	57.9	1860-1955 bp	1.0	82.3
trnE-UUC-trnT-GGU_p3	TAGATTGGCGAATTGTGGGG	TCGACAAATTCTTGCGGAGT	50.0	45.0	57.9	57.8	1603-1698 bp	1.0	82.0
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CGCATCCATTCTCTATCGCA	50.0	50.0	57.9	57.9	1668-1769 bp	1.0	81.6
trnE-UUC-trnT-GGU_p5	ATCAGATTGCCTTTCCGCTT	CGCATCCATTCTCTATCGCA	45.0	50.0	57.9	57.9	2288-2292 bp	0.75	68.8