Hotspot

psbM-trnD-GUC

LSC · 30801-31401 · 601 bp

Back to Ceropegia

Pi 0.0491
MarkerSeek score 71.80
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
601
variable_sites
59
pi
0.0491
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGCGGTAGGAACTAGAACG TTCAATTGGTCAGAGCACCG 55.0 50.0 58.9 58.5 744-789 bp 1.0 76.4
psbM-trnD-GUC_p2 GGCGGTAGGAACTAGAACGA TTCAATTGGTCAGAGCACCG 55.0 50.0 58.9 58.5 743-788 bp 1.0 76.4
psbM-trnD-GUC_p3 GCGGTAGGAACTAGAACGAA TTCAATTGGTCAGAGCACCG 50.0 50.0 57.1 58.5 742-787 bp 1.0 76.2
psbM-trnD-GUC_p4 AGAAAGGCGGTAGGAACTAGA TTCAATTGGTCAGAGCACCG 47.6 50.0 57.9 58.5 748-793 bp 1.0 74.8
psbM-trnD-GUC_p5 AGTAGAAAGGCGGTAGGAACT TTCAATTGGTCAGAGCACCG 47.6 50.0 58.2 58.5 751-796 bp 1.0 74.1