Hotspot

trnS-GCU-trnG-UCC

LSC · 8627-9129 · 503 bp

Back to Ceropegia

Pi 0.0129

MarkerSeek score 63.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 13
pi: 0.0129
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TGCAAATAAAGTTTTTGGTCGGA	45.0	34.8	58.0	57.7	624-657 bp	1.0	59.2
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	550-583 bp	1.0	57.2
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	TGCAAATAAAGTTTTTGGTCGGA	50.0	34.8	57.6	57.7	610-643 bp	1.0	56.3
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	TGCAAATAAAGTTTTTGGTCGG	45.0	36.4	58.0	56.3	624-657 bp	1.0	56.0
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	TGCAAATAAAGTTTTTGGTCGGA	55.0	34.8	58.5	57.7	605-638 bp	1.0	55.4