Hotspot

rpl16

LSC · 91480-93120 · 1641 bp

Back to Ceriops

Pi 0.0433

MarkerSeek score 76.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1641
variable_sites: 97
pi: 0.0433
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTTTGGCTCCGCTGTTATC	ATCTTCCGGTCTTGAGGGAA	50.0	50.0	58.0	58.0	2463-2512 bp	0.666667	70.1
rpl16_p2	TCTTTGGCTCCGCTGTTATC	TCTTCCGGTCTTGAGGGAAT	50.0	50.0	58.0	58.0	2462-2511 bp	0.666667	70.1
rpl16_p3	CTTTGGCTCCGCTGTTATCT	ATCTTCCGGTCTTGAGGGAA	50.0	50.0	58.0	58.0	2462-2511 bp	0.666667	70.1
rpl16_p4	CTTTGGCTCCGCTGTTATCT	TCTTCCGGTCTTGAGGGAAT	50.0	50.0	58.0	58.0	2461-2510 bp	0.666667	70.1
rpl16_p5	ATCGATGACAACTGCAGCAT	TCTTCCGGTCTTGAGGGAAT	45.0	50.0	58.0	58.0	2673-2722 bp	0.666667	69.8