Hotspot

rpl14-rpl16

LSC · 91347-91479 · 133 bp

Back to Ceriops

Pi 0.053

MarkerSeek score 70.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 10
pi: 0.053
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCTTTGGCTCCGCTGTTATC	AGAAACACGTATGGGTTCGG	50.0	50.0	58.0	57.9	356-364 bp	1.0	83.1
rpl14-rpl16_p2	TCTTTGGCTCCGCTGTTATC	AGAACGCGATTCCGTAAACA	50.0	45.0	58.0	57.9	577-585 bp	1.0	82.0
rpl14-rpl16_p3	CTTTGGCTCCGCTGTTATCT	AGAACGCGATTCCGTAAACA	50.0	45.0	58.0	57.9	576-584 bp	1.0	82.0
rpl14-rpl16_p4	GCGTCTTTGATCACAGCAAC	AGAACGCGATTCCGTAAACA	50.0	45.0	58.0	57.9	661-669 bp	1.0	81.7
rpl14-rpl16_p5	ATCGATGACAACTGCAGCAT	AGAACGCGATTCCGTAAACA	45.0	45.0	58.0	57.9	788-796 bp	1.0	81.4