Hotspot

trnS-UGA-psbZ

LSC · 41559-42014 · 456 bp

Back to Ceriops

Pi 0.0526

MarkerSeek score 79.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 29
pi: 0.0526
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	533-714 bp	1.0	87.2
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	CGACCAACCATCAGGAGAAG	55.0	55.0	58.1	58.0	522-703 bp	1.0	86.7
trnS-UGA-psbZ_p3	CAAGACCGGAGCTATCAACC	TACTCGACCAACCATCAGGA	55.0	50.0	58.1	57.8	537-718 bp	1.0	85.5
trnS-UGA-psbZ_p4	CTATCAACCACTCGGCCATC	TACTCGACCAACCATCAGGA	55.0	50.0	58.1	57.8	526-707 bp	1.0	85.0
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	TCGACCAACCATCAGGAGAA	55.0	50.0	58.1	58.4	534-715 bp	1.0	84.4