Hotspot

trnT-GGU-psbD

LSC · 36633-38812 · 2180 bp

Back to Ceriops

Pi 0.0441

MarkerSeek score 73.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 2180
variable_sites: 117
pi: 0.0441
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TTTCCCAAGGGCTATAGTCA	50.0	45.0	58.3	55.5	2100-2237 bp	1.0	66.1
trnT-GGU-psbD_p2	GGCCCTTTTAACTCAGTGGT	TTTCCCAAGGGCTATAGTCA	50.0	45.0	57.7	55.5	2138-2275 bp	1.0	66.0
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TTCCCAAGGGCTATAGTCAT	50.0	45.0	58.3	55.3	2099-2236 bp	1.0	64.2
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	TTCCCAAGGGCTATAGTCAT	50.0	45.0	57.7	55.3	2137-2274 bp	1.0	64.1
trnT-GGU-psbD_p5	AGGCCCTTTTAACTCAGTGG	TTTCCCAAGGGCTATAGTCA	50.0	45.0	57.4	55.5	2139-2276 bp	1.0	63.6