Hotspot

trnE-UUC-trnT-GGU

LSC · 35295-36558 · 1264 bp

Back to Ceriops

Pi 0.036
MarkerSeek score 76.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1264
variable_sites
61
pi
0.036
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 1249-1467 bp 1.0 85.4
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 1228-1446 bp 1.0 85.3
trnE-UUC-trnT-GGU_p3 TCGTAATACCCTACCCCCAG GAACCGATGACTTACGCCTT 55.0 50.0 57.6 58.0 1285-1503 bp 1.0 84.3
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.3 58.2 1246-1464 bp 1.0 84.3
trnE-UUC-trnT-GGU_p5 TGTCCTGAACCACTAGACGA CCGATGACTTACGCCTTACC 50.0 55.0 57.7 58.2 1225-1443 bp 1.0 84.2