Hotspot

psbI-trnS-GCU

LSC · 8099-8269 · 171 bp

Back to Ceriops

Pi 0.0429

MarkerSeek score 72.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 10
pi: 0.0429
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATGATCCGGGGCGCAAT	TGGACTAAAGCGTCGGATTG	55.6	50.0	59.8	58.0	280-483 bp	1.0	55.0
psbI-trnS-GCU_p2	AATGATCCGGGGCGCAAT	GTGGACTAAAGCGTCGGATT	55.6	50.0	59.8	58.0	281-484 bp	1.0	54.9
psbI-trnS-GCU_p3	AATGATCCGGGGCGCAATC	TGGACTAAAGCGTCGGATTG	57.9	50.0	60.9	58.0	280-483 bp	1.0	54.2
psbI-trnS-GCU_p4	AATGATCCGGGGCGCAATC	GTGGACTAAAGCGTCGGATT	57.9	50.0	60.9	58.0	281-484 bp	1.0	54.2
psbI-trnS-GCU_p5	AATGATCCGGGGCGCAAT	ATTGGGAGAGATGGCTGAGT	55.6	50.0	59.8	58.1	299-502 bp	1.0	53.8