Hotspot

trnK-UUU-trnQ-UUG

LSC · 5047-6674 · 1628 bp

Back to Ceriops

Pi 0.0532

MarkerSeek score 86.20

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1628
variable_sites: 112
pi: 0.0532
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1491-1952 bp	1.0	87.3
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1490-1951 bp	1.0	87.3
trnK-UUU-trnQ-UUG_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1477-1938 bp	1.0	85.0
trnK-UUU-trnQ-UUG_p4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	1502-1963 bp	1.0	84.0
trnK-UUU-trnQ-UUG_p5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	1500-1961 bp	1.0	82.8