Hotspot

rps19

LSC · 86754-87032 · 279 bp

Back to Cercocarpus

Pi 0.00239

MarkerSeek score 28.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 1
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1117-1134 bp	1.0	80.7
rps19_p2	TTGTCCCATAATCCCCCACT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	1025-1042 bp	1.0	80.4
rps19_p3	TTGTCCCATAATCCCCCACT	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.0	58.1	1305-1322 bp	1.0	80.1
rps19_p4	TTGTCCCATAATCCCCCACT	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.0	58.1	1296-1313 bp	1.0	80.0
rps19_p5	TTGTCCCATAATCCCCCACT	GTTTGGGTAAAGCCGGATCT	50.0	50.0	58.0	57.9	1219-1236 bp	1.0	80.0