Hotspot

rpl16

LSC · 83908-85313 · 1406 bp

Back to Cercocarpus

Pi 0.00237

MarkerSeek score 60.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1406
variable_sites: 5
pi: 0.00237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTTTTTCAATGCAAAGGGC	GCAAACTCTTTCATTCTTTTTCTGT	40.0	32.0	55.3	57.2	1618-1622 bp	1.0	40.4
rpl16_p2	TTCTTTTTCAATGCAAAGGGC	GCAAACTCTTTCATTCTTTTTCTGT	38.1	32.0	56.1	57.2	1619-1623 bp	1.0	40.4
rpl16_p3	TCTTTTTCAATGCAAAGGGC	AGCAAACTCTTTCATTCTTTTTCTG	40.0	32.0	55.3	57.0	1619-1623 bp	1.0	40.4
rpl16_p4	ACCTCTTTCCTTTGGTTCTACA	GCAAACTCTTTCATTCTTTTTCTGT	40.9	32.0	56.9	57.2	1524-1529 bp	1.0	40.4
rpl16_p5	TCTTTTTCAATGCAAAGGGC	GCAAACTCTTTCATTCTTTTTCTGTT	40.0	30.8	55.3	57.8	1618-1622 bp	1.0	40.4