Hotspot

ndhE-ndhG

SSC · 121187-121431 · 245 bp

Back to Cercocarpus

Pi 0.00544

MarkerSeek score 56.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 245
variable_sites: 2
pi: 0.00544
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTTCGGCTCGTGATCAATCC	TTGATAGGTGCAATTGCCGT	50.0	45.0	58.3	58.2	352 bp	1.0	76.3
ndhE-ndhG_p2	CGGCTCGTGATCAATCCATA	TTGATAGGTGCAATTGCCGT	50.0	45.0	57.6	58.2	349 bp	1.0	75.6
ndhE-ndhG_p3	TTTCGGCTCGTGATCAATCC	AGTCGCTTTGATAGGTGCAA	50.0	45.0	58.3	57.5	359 bp	1.0	73.9
ndhE-ndhG_p4	AACCATATTTCGGCTCGTGA	TTGATAGGTGCAATTGCCGT	45.0	45.0	57.3	58.2	359 bp	1.0	73.6
ndhE-ndhG_p5	CGGCTCGTGATCAATCCATA	AGTCGCTTTGATAGGTGCAA	50.0	45.0	57.6	57.5	356 bp	1.0	73.2