Hotspot

ndhF-rpl32

SSC · 115542-116357 · 816 bp

Back to Cercocarpus

Pi 0.00408

MarkerSeek score 49.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 816
variable_sites: 5
pi: 0.00408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	AAAAAGCTTTCAACGCTGCC	40.0	45.0	57.0	58.4	1013-1015 bp	1.0	68.9
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	TTCAACGCTGCCCAATATCC	40.0	50.0	57.0	58.6	1005-1007 bp	1.0	67.4
ndhF-rpl32_p3	TTTGTTGCCGTTGGAAAAAGT	AAAAAGCTTTCAACGCTGCC	38.1	45.0	57.7	58.4	1014-1016 bp	1.0	66.6
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	ACGAAAAAGCTTTCAACGCT	40.0	40.0	57.0	57.2	1016-1018 bp	1.0	65.6
ndhF-rpl32_p5	CCACATACGGCGAAGATTTT	AAAAAGCTTTCAACGCTGCC	45.0	45.0	56.5	58.4	1033-1035 bp	1.0	65.3