Hotspot

rps19-rpl2

IRb · 87033-87110 · 78 bp

Back to Cercocarpus

Pi 0.0183

MarkerSeek score 60.20

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 78
variable_sites: 2
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCTGCTTTTGTGTTAAGTGT	TTGATTCTTCGTCGCCGTAG	35.0	50.0	53.3	58.1	175-185 bp	1.0	46.3
rps19-rpl2_p2	TTCTGCTTTTGTGTTAAGTGT	TTGATTCTTCGTCGCCGTAG	33.3	50.0	54.2	58.1	176-186 bp	1.0	45.4
rps19-rpl2_p3	TCTGCTTTTGTGTTAAGTGT	GGGCCCCAATTGGTAGAAAA	35.0	50.0	53.3	58.1	267-277 bp	1.0	45.2
rps19-rpl2_p4	TTCTGCTTTTGTGTTAAGTGT	GGGCCCCAATTGGTAGAAAA	33.3	50.0	54.2	58.1	268-278 bp	1.0	44.4
rps19-rpl2_p5	TCTGCTTTTGTGTTAAGTGT	TTGGGGTTATCCTGCACTTG	35.0	50.0	53.3	57.8	233-243 bp	1.0	44.4