Hotspot

rpl20-rps12

LSC · 71463-72245 · 783 bp

Back to Cercocarpus

Pi 0.0017
MarkerSeek score 47.30
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
783
variable_sites
2
pi
0.0017
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 904-907 bp 1.0 78.7
rpl20-rps12_p2 TGAATGAGCCCCTCGAAAAG CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 902-905 bp 1.0 78.7
rpl20-rps12_p3 GAATGAGCCCCTCGAAAAGT CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 901-904 bp 1.0 78.7
rpl20-rps12_p4 GAGCCCCTCGAAAAGTTGAT CTCAACGACGAGGAACATGT 50.0 50.0 57.9 57.9 897-900 bp 1.0 78.7
rpl20-rps12_p5 AGCCCCTCGAAAAGTTGATG CTCAACGACGAGGAACATGT 50.0 50.0 58.2 57.9 896-899 bp 1.0 78.3