Hotspot

rpl33-rps18

LSC · 70323-70530 · 208 bp

Back to Cercocarpus

Pi 0.00641
MarkerSeek score 41.20
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
208
variable_sites
2
pi
0.00641
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TACGCCTAGTCGATTGGAGT TCAATTCGATCCCCCGATTG 50.0 50.0 58.0 57.7 373 bp 1.0 78.0
rpl33-rps18_p2 TACGCCTAGTCGATTGGAGT ATTCGATCCCCCGATTGGAT 50.0 50.0 58.0 58.6 370 bp 1.0 74.9
rpl33-rps18_p3 CGCCTAGTCGATTGGAGTTG TCAATTCGATCCCCCGATTG 55.0 50.0 58.4 57.7 371 bp 1.0 74.9
rpl33-rps18_p4 TACGCCTAGTCGATTGGAGT CAATTCGATCCCCCGATTGG 50.0 55.0 58.0 58.8 372 bp 1.0 74.0
rpl33-rps18_p5 TACGCCTAGTCGATTGGAGT AATTCGATCCCCCGATTGGA 50.0 50.0 58.0 58.9 371 bp 1.0 73.1