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psbE-petL

LSC · 66981-68269 · 1289 bp

Back to Cercocarpus

Pi 0.00103

MarkerSeek score 59.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 2
pi: 0.00103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TCGTATTTTGCTCAGACCGA	50.0	45.0	57.8	57.0	1403-1406 bp	1.0	70.7
psbE-petL_p2	ACGAATAACCAACCCGCAAT	TCGTATTTTGCTCAGACCGA	45.0	45.0	58.2	57.0	1489-1492 bp	1.0	70.6
psbE-petL_p3	TGCTGACGAATAACCAACCC	TCGTATTTTGCTCAGACCGA	50.0	45.0	58.2	57.0	1494-1497 bp	1.0	70.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TCGTATTTTGCTCAGACCGA	55.0	45.0	58.2	57.0	1398-1401 bp	1.0	70.4
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GTCGTATTTTGCTCAGACCG	50.0	50.0	57.8	56.9	1404-1407 bp	1.0	70.3