Hotspot

petA-psbJ

LSC · 65331-66209 · 879 bp

Back to Cercocarpus

Pi 0.00986

MarkerSeek score 55.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 13
pi: 0.00986
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	1015-1023 bp	1.0	78.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.0	57.4	1014-1022 bp	1.0	78.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1012-1020 bp	1.0	77.4
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1011-1019 bp	1.0	76.9
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1010-1018 bp	1.0	76.0